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Requirement of Carbohydrates for Cell Fusion Induced by Vesicular Stomatitis Virus
Author(s) -
Takehara Manabu
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00039.x
Subject(s) - vesicular stomatitis virus , biology , sodium azide , cell fusion , virus , mannose , dinitrophenol , virology , vesicular stomatitis , cell culture , biochemistry , cell , genetics
The fusion of BHK‐21‐KB cells by vesicular stomatitis virus was not induced in Eagle's minimal essential medium without glucose. In medium containing glucose, the rate of polykaryocyte formation decreased as the concentration of glucose was reduced below 5 mM. However, no reduction in virus production 24 hr after infection was seen under this condition. Addition of pyruvate or mannose to the culture medium caused a reversal of cell fusing activity. Cell fusion and virus growth were significantly suppressed by sodium azide and 2,4‐dinitrophenol.