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On the Immunogenicity of Ribosomes and Ribosomal Proteins Isolated from Klebsiella pneumoniae and Streptococcus pneumoniae
Author(s) -
Robert Daniel,
Michel Serge,
Ivanoff Bernard,
Cozzone Alain J.,
Fontanges Robert
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00021.x
Subject(s) - ribosome , klebsiella pneumoniae , biology , ribosomal protein , microbiology and biotechnology , ribosomal rna , immunogenicity , streptococcus pneumoniae , puromycin , escherichia coli , biochemistry , protein biosynthesis , antibiotics , antigen , gene , rna , genetics
Abstract The two pathogenic species Streptococcus pneumoniae and Klebsiella pneumoniae were used to analyze the immunogenic role of proteins in ribosomal preparations. The protective activity of ribosomes prepared from either strain and further purified by washing with high‐salt concentrations, followed or not by sucrose gradient separation of the particles, was identical to that of crude unwashed ribosomes. Similarly, no substantial alteration of the level of protection was observed after treatment with the antibiotic puromycin. Therefore, the immunizing efficacy of ribosomes does not appear to be due either to the nonribosomal proteins adsorbed at the surface of organelles or to the growing polypeptide chain. It seems rather to be attributable to the structural ribosomal proteins themselves, which were indeed shown to induce alone a significant level of protection.