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Close Association of Virus‐Specific Cell Surface (S) and H‐2 Antigens in Ad12‐Infected and ‐Transformed Mouse Cells
Author(s) -
Maeta Yasunobu,
Hamada Chuya
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00017.x
Subject(s) - antigen , ctl* , biology , microbiology and biotechnology , cytotoxic t cell , lysis , antibody , virology , virus , cell , cell culture , immunology , in vitro , cd8 , biochemistry , genetics
A virus‐specific cell surface (S) antigen in adenovirus type 12 (Ad12)‐transformed mouse cells has been assumed to be a direct target for cytotoxic thymus‐derived lymphocytes (CTL). In this study, the spatial proximity between the S and H‐2 antigens was determined by three different methods, the proximity and co‐capping tests, and the test for blocking of CTL‐mediated lysis by anti‐H‐2 serum. In the proximity test with Ad12‐infected thymic and splenic lymphocytes, and an Ad12‐transformed line of C3H/He (H‐2 k ) mouse cells, anti‐H‐2 k and anti‐S sera reciprocally inhibited fluorescent‐antibody staining of the opposite antigens. By contrast, anti‐Thy‐1, 2 serum as well as anti‐Ia and anti‐Ig sera failed to show any appreciable effect in this test, when paired with anti‐S serum. In addition, the S and H‐2 antigens co‐capped in the infected thymic lymphocytes, and CTL‐mediated lysis of the transformed cells was abrogated equally by treatment of cells with anti‐S and anti‐H‐2 sera. These results clearly demonstrate that there is a close proximity between the S and H‐2 antigens on the surface of Ad12‐infected and ‐transformed mouse cells.