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Formation of Mononuclear Phagocyte (Macrophage) Colonies by Mouse Spleen Cells in Liquid Culture
Author(s) -
Yokochi Takashi,
Nakashima Izumi,
Ohta Michio,
Hasegawa Takaaki,
Fujii Yasuaki,
Kato Nobuo
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00004.x
Subject(s) - mononuclear phagocyte system , phagocyte , biology , macrophage , spleen , immunology , peripheral blood mononuclear cell , liquid culture , microbiology and biotechnology , phagocytosis , in vitro , genetics , botany
Abstract The effect of the capsular polysaccharide of Klebsiella pneumoniae type 1 Kasuya strain (CPS‐K) on the formation of macrophage colonies in cultures of mouse spleen cells was investigated by the liquid culture technique during an incubation period of 7–8 days. CPS‐K markedly inhibited further generation of macrophage colonies when added at any time after the beginning of culture, whereas it showed no destructive effect on macrophage colonies which were already formed before its addition. When CPS‐K was present throughout the incubation period, such a low concentration as 0.05 μ g/ml significantly inhibited colony formation, and the intensity of its inhibitory effect depended on its dose in the range of 0.005–50 μ g/ml. The inhibitory effect persisted even if CPS‐K was washed out after spleen cells were kept in contact with 20 μ g of CPS‐K per ml at 37 C for 6 hr. It was found that the inhibitory effect of CPS‐K on colony formation was not mediated through its action on T cells, B cells or macrophages, and that it was not due to the generation of suppressor cells capable of inhibiting colony formation. It is concluded therefore that CPS‐K directly inhibits the proliferation of macrophage colony‐forming cells. The active substance responsible for the inhibitory effect of CPS‐K on colony formation is the neutral polysaccharide fraction of CPS‐K.