Premium
Effects of a Bacteriocin from Mycobacterium smegmatis on BALB/3T3 and Simian Virus 40‐Transformed BALB/c Mouse Cells
Author(s) -
Saito Hajime,
Watanabe Takashi
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00002.x
Subject(s) - bacteriocin , biology , mycobacterium smegmatis , microbiology and biotechnology , 3t3 cells , cell culture , virology , transfection , mycobacterium tuberculosis , medicine , tuberculosis , pathology , genetics , antimicrobial
The effects of a bacteriocin from Mycobacterium smegmatis ATCC 14468 on simian virus 40‐transformed BALB/c mouse cells (mKS‐A TU‐7 cells) and non‐transformed BALB/3T3 cells originating from the BALB/c mouse strain were studied. The percentage of nigrosin‐unstained (viable) cells in the bacteriocin‐treated mKS‐A TU‐7 cells decreased time‐dependently with an increase in the bacteriocin activity. There was a time‐dependent decrease in the bacteriocin activity after treatment with the cell membrane preparation from mKS‐A TU‐7 cells. There was no apparent effect of the bacteriocin on the viability of non‐transformed BALB/3T3 cells. Wheat germ agglutinin blocked the toxic effect of bacteriocin on mKS‐A TU‐7 cells. These results indicate that the higher sensitivity and binding capacity of the tumor cells to the bacteriocin is probably due to the presence of a larger amount of N ‐acetyl‐glucosamine or closely related sugar residues with a high affinity for bacteriocin, as compared with normal cells. The bacteriocin produced morphological alterations and inhibition of synthesis of ribonucleic acid, deoxyribonucleic acid and protein in the transformed but not in the nontransformed cells.