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A Complement Inhibitor Produced by Stachybotrys complementi , nov. sp. K‐76, a New Species of Fungi Imperfecti
Author(s) -
Miyazaki Wasei,
Tamaoka Hisashi,
Shinohara Masanao,
Kaise Hirotsugu,
Izawa Taketoshi,
Nakano Yoshimasa,
Kinoshita Taroh,
Hong Kyongsu,
Inoue Kozo
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02914.x
Subject(s) - biology , fungi imperfecti , complement (music) , microbiology and biotechnology , botany , genetics , complementation , gene , phenotype
A complement inhibitor, K‐76, was isolated and purified from the culture supernatant of a fungus, Stachybotrys complementi , nov. sp. K‐76, isolated from soil of Ishigaki Island, Okinawa. K‐76 is a sesquiterpene compound and it can be oxidized to a monocarboxylic derivative (K‐76 COOH), the sodium salt of which is very soluble and much less toxic than K‐76. K‐76 and K‐76 COOH both inhibited complement activation by either the classical or alternative pathway. They inhibited generation of the factor chemotactic to human polymorphonuclear leukocytes from human serum by aggregated immunoglobulin. When sensitized erythrocytes were treated with complement in the presence of K‐76 COOH, the resulting unlysed cells were found to be in the state of EAC1, 4b, 2a, 3b. Thus K‐76 COOH is considered to block mainly the C5 intermediate step. K‐76 COOH did not inhibit any proteases or esterases tested, except when tested at high concentration.