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Studies on the Neutralization of Herpes Simplex Virus
Author(s) -
Yoshino Kamesaburo,
Urayama Mari,
Abe Kazuko
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02912.x
Subject(s) - antibody , sensitization , herpes simplex virus , neutralization , virology , immunology , neutralizing antibody , biology , immunoglobulin g , immunoglobulin m , virus
Late IgG and IgM from a rabbit immunized with herpes virus were tested for ordinary neutralizing (N) antibody, complement‐requiring neutralizing (CRN) antibody and in addition CRN antibody detectable by overnight sensitization at 0 C (s‐CRN antibody). Heat stability tests showed that IgG s‐CRN antibody was slightly less resistant to heating at 70 C than were N and CRN antibodies, whereas all three activities of IgM were quickly degraded at this temperature. Dose‐response curves with varying amounts of complement (C) or anti‐antibody revealed a marked difference between IgG s‐CRN and IgM s‐CRN antibodies. While 1‐hr sensitization at 37 C was insufficient to detect IgG s‐CRN antibody, it had the same effect as overnight sensitization at 0 C for IgM s‐CRN antibody. When sensitization at 0 C was prolonged to 3 days, unexpectedly high endpoints exceeding 1:10,000 were obtained even with IgM. Consequently, enhancement by C was several hundred‐fold with IgM in contrast to 5‐ to 10‐fold enhancement of IgG s‐CRN antibody, which was similar to that after overnight sensitization. Also IgM obviously required more C than did IgG. These results suggest that IgM of late serum is slower reacting and more C‐dependent than IgG s‐CRN antibody. Tests with early rabbit sera indicated that the s‐CRN antibody detectable by 3‐day sensitization reaches a high level before the appearance of N antibody.