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Isolation and Characterization of Temperature‐Sensitive Mutants of Sendai Virus
Author(s) -
Adachi Akio,
Kanda Tadahito,
Shibuta Hiroshi
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02911.x
Subject(s) - complementation , sendai virus , mutant , biology , microbiology and biotechnology , hemagglutinin (influenza) , virology , neuraminidase , rna , mutagenesis , virus , gene , biochemistry
Sixteen temperature‐sensitive mutants of Sendai virus were isolated from mutagenized stocks (10 mutants, designated numerically) and persistently infected cultures (6 mutants, designated alphabetically). Based on complementation tests, virion‐associated activities, thermal inactivation, and viral RNA and hemadsorbing antigen synthesis as well as virion production in chick lung embryo cells at nonpermissive temperature, these mutants were divided into seven groups as follows. HANA group mutants (ts−5, −9, −10,−201), defective in hemagglutinin‐neuraminidase protein, complementation group I. F group mutants (ts‐18, −108), defective in hemolytic and cell‐fusing activity, complementation group II. Ts‐43, defective in RNA polymerase activity, complementation group III. Ts‐23, defective in RNA polymerase activity, interfered with the other mutants in complementation tests. Ts‐25, defective in the incorporation of hemagglutinin‐neuraminidase protein into the virion at the stage of virus assembly. Ts‐110, belongs to F group mutants on one hand, but is considered to carry another undetermined defect. C group (carrier culture‐borne group) mutants (ts‐a, ‐b, ‐c, ‐d, ‐e, ‐f), defective lesion not yet determined and belong to neither complementation group I nor II. Assignment of mutants in groups iv), v), vi), and vii) to complementation groups could not be achieved.