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Enhanced Production of Virus‐Inhibiting Factor (Interferon) in Human Diploid Cells by Ultraviolet Irradiation and Temperature Shift‐Down after Stimulation with Newcastle Disease Virus
Author(s) -
Maehara Nobutoshi,
Komatsu Haruko,
Shimoda Kazunobu,
Marino Satoshi,
Nagano Yasuiti,
Matumoto Minoru
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02896.x
Subject(s) - incubation , newcastle disease , biology , virus , stimulation , interferon , incubation period , cell culture , inoculation , ultraviolet light , virology , microbiology and biotechnology , biochemistry , immunology , chemistry , endocrinology , genetics , photochemistry
The production of the virus‐inhibiting factor or interferon (IF) was highest in cells incubated at 37 C after inoculation with Newcastle disease (ND) virus and decreased as the incubation temperature was lowered. Shift‐down of incubation temperature to 32 C or 34 C after incubation at 37 C for 4–7 hr enhanced IF production in cell cultures stimulated with ND virus, as compared with cultures incubated continuously at 37 C. Shift‐down to 32 C after incubation at 37 C for 6 hr. was optimal for this enhancement of IF yield. Enhanced IF production was also observed in cell cultures irradiated by ultraviolet light 4–7 hr after stimulation with ND virus.