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Increased Nitroblue Tetrazolium Dye Reduction by Human Neutrophils Stimulated by Interferon In Vitro
Author(s) -
Pak ChinBin,
Imanishi Jiro,
Kishida Tsunataro,
Matsuo Akio
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02872.x
Subject(s) - nitroblue tetrazolium , interferon , in vitro , formazan , biology , immunology , microbiology and biotechnology , chemistry , biochemistry
Human leukocyte interferon enhanced nitroblue tetrazolium dye (NBT) reduction by human neutrophils (PMNs). Increase in NBT reduction paralleled increase in interferon dose. When human leukocyte interferon was heated to 60 C or 80 C for 30 min, both the antiviral activity and the effect on NBT reduction decreased. Human leukocyte interferon neutralized with anti‐human leukocyte interferon serum showed no effect on NBT reduction. A human fibroblast interferon preparation also enhanced NBT reduction. The species dependency of interferon was shown in NBT reduction as well as in antiviral activity.