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Sugar Composition of O‐Antigenic Lipopolysaccharides Isolated from Vibrio parahaemolyticus
Author(s) -
Hisatsune Kazuhito,
Kiuye Akira,
Kondo Seiichi
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02870.x
Subject(s) - vibrio parahaemolyticus , chemotype , biology , sugar , sugar acids , microbiology and biotechnology , agglutination (biology) , biochemistry , amino sugar , galactose , arabinose , bacteria , antigen , food science , xylose , fermentation , genetics , essential oil
Vibrio parahaemolyticus , a causative bacterium of food poisoning unique for its particular primary association with sea products, is now divided serologically into 11 or 12 O‐forms based on agglutination and agglutinin‐absorption tests. We determined the sugar composition of the somatic O‐antigens, i.e ., lipopolysaccharides (LPS), of representative strains of each O‐form. Of particular interest is the absence of evidence for the presence of 2‐keto‐3‐deoxy‐octonic acid (KDO), a regular sugar component of gram‐negative bacterial LPS, in any LPS examined, with the exception of 06. Furthermore, 07 and 012 LPS contained a KDO‐like compound that is, however, not identical with KDO. Glucose, glucosamine, and L‐glycero‐D‐mannoheptose were found as common sugar constituents. Three unidentified amino sugars, designated here as P1, P2, and P3, were found. Various combinations of each of these unidentified amino sugars, and of galactose, fucose, arabinose, D‐glycero‐D‐mannoheptose, galactosamine, KDO, and the KDO‐like substance were detected in accordance with the O‐form of LPS. On the basis of the sugar composition, LPS of the 12 O‐forms of V. parahaemolyticus can be classified into nine chemotypes, because 03, 05, and 011 LPS belong to the same chemotype and 07 and 012 to another chemotype.