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Effect of Oxidizing Agents and Sulfhydryl Group Reagents on Beta Toxin from Clostridium perfringens Type C
Author(s) -
Sakurai Jun,
Fujii Yoshio,
Matsuura Miho
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02862.x
Subject(s) - dithiothreitol , dtnb , iodoacetic acid , toxin , iodoacetamide , clostridium perfringens , biochemistry , ferricyanide , glutathione , thiol , oxidizing agent , microbiology and biotechnology , biology , incubation , cysteine , chemistry , enzyme , bacteria , organic chemistry , genetics
Purified beta toxin from Clostridium perfringens type C was inactivated by the oxidizing agents o ‐iodosobenzoate (OIBA), oxidized glutathione, and ferricyanide, and by the sulfhydryl group reagents 5,5′‐dithio‐bis(2‐nitrobenzoic acid) (DTNB) and N ‐ethylmaleimide, iodoacetamide, and iodoacetic acid, causing loss of activity in various degrees depending on the concentration used. The activity of the toxin was not influenced by exposure to 1.0 mM of p ‐chloromercuribenzoate. The toxin treated by OIBA or DTNB was reactivated by incubation with 2‐mercaptoethanol and dithiothreitol. The data suggest that beta toxin contains thiol groups which are essential for the activity.