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Studies on Structural Proteins of Chikungunya Virus
Author(s) -
Konishi Eiji,
Hotta Susumu
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02846.x
Subject(s) - chikungunya , coomassie brilliant blue , polyacrylamide gel electrophoresis , biology , staining , sodium dodecyl sulfate , phosphate buffered saline , tris , gel electrophoresis , buffer (optical fiber) , chromatography , acrylamide , polyacrylamide , sodium , microbiology and biotechnology , virus , buffer solution , virology , biochemistry , chemistry , monomer , genetics , polymer , telecommunications , computer science , enzyme , organic chemistry
Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate‐polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E 1 and E 2 ) were associated with the membrane, and one (C) with the core. Their molecular weights were estimated to be 56,000 (E 1 ), 52,000 (E 2 ), and 36,000 (C), irrespective of the concentration of acrylamide in the gels. The molar ratios of Ei, E2, and C were almost equal when the sample buffer was Tris‐HCl, whereas they were different when phosphate buffer was used.