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Electron Microscopic Observation of New Transposable Elements Inserted into P22 Phage Genome from R Plasmids
Author(s) -
Suzuki Kenji,
Mise Katsutoshi,
Nakaya Rintaro
Publication year - 1980
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1980.tb02834.x
Subject(s) - transposable element , biology , genome , heteroduplex , tn3 transposon , plasmid , sleeping beauty transposon system , genetics , transposition (logic) , dna , gene , linguistics , philosophy
By using phage P22spl, a deletion mutant of phage P22, the structures of two new transposons on P22 genomes were studied by the electron microscopic heteroduplex method. One of these was the Cm (chloramphenicol) transposon derived from an R plasmid, NR1, and the other the Km (kanamycin) transposcn from pNR502. The heteroduplex between P22 phage DNAs with and without the Cm transposon revealed that the Cm transposon was similar in structure to the Tn 9 element, a well‐known Cm transposon derived from the R plasmid pMS14. On the other hand, the Km transposon of pNR502 was quite different in structure from other Km transposons reported previously. This transposon consists of a 6.8 kilo‐base (kb) segment of DNA, in which a short inverted repeat is contained. The heteroduplex experiments showed that a 4.5 kb segment of DNA was deleted from the P22 genome in the P22spl genome. Because of a shorter unit length of the genome, phage P22spl is considered to be useful for assaying various kinds of transposable elements.