Premium
Isolation and Characterization of Heat‐Resistant (HR) Mutants of Newcastle Disease Virus 1
Author(s) -
Iinuma Masao,
Nishiyama Yukihiro,
Hamaguchi Michinari,
Yoshida Tetsuya,
Nagai Yoshiyuki,
Maeno Koichiro,
Matsumoto Toshisada
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00550.x
Subject(s) - biology , virus , infectivity , newcastle disease , mutant , virology , multiplicity of infection , glycoprotein , hela , cell culture , wild type , microbiology and biotechnology , infectious bursal disease , in vitro , virulence , biochemistry , gene , genetics
Heat‐resistant (HR) mutants (HR 70 and HR 74) of Newcastle disease virus (NDV) which exhibited significantly higher thermostability in their infectivity than wild‐type virus were isolated and characterized. They differ from each other in their plaque morphology; HR 70 produces small turbid plaques, whereas those of HR 74 are large and clear. Cytopathogenicity of these mutants is much lower than that of wild‐type virus in cultured cells such as CEF, LLCMK 2 , and HeLa cells. Moreover, these HR mutants exhibited extended mean embryo survival times. Synthesis of cellular RNAs and proteins in cells infected with HR mutants was not significantly reduced under conditions in which synthesis of these macromolecules was strongly reduced in cells infected with wild‐type virus. No significant differences were observed between HR mutants and wild‐type virus in their other phenotypic characteristics such as the capacity for interferon production, growth characteristics at a low multiplicity of infection, and cleavage of viral glycoproteins in infected cells. From these findings, it was suggested that the inhibitory effect of virus infection on cellular macromolecular synthesis is a possible determinant of cytopathogenicity of NDV.