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Regulatory Mechanism of Reagin Production in Mice at the T Cell Level
Author(s) -
Takatsu Kiyoshi,
Tominaga Akira,
Hamaoka Toshiyuki,
Kitagawa Masayasu
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00542.x
Subject(s) - biology , immunology , adoptive cell transfer , antibody , immunoglobulin e , t cell , microbiology and biotechnology , cytotoxic t cell , cd40 , b cell , antigen , interleukin 21 , immune system , in vitro , biochemistry
Helper T cell activities specific for purified protein derivative (PPD) generated by immunization with Mycobacterium tuberculosis (Tbc) or PPD were investigated concerning adoptive IgE and IgG antibody responses. It is interesting that preferential triggering activity of IgG antibody response was observed when PPD‐reactive cells from mice immunized with Tbc were used as a helper cell source. The selective triggering of IgG B cells by Tbc‐primed cells was consistently observed using DNP‐primed B cell populations from mice immunized with DNP‐carrier conjugate in either ICFA or alum. T cell dependency of helper activity was demonstrated by the fact that treatment of Tbc‐primed cells with anti‐Thy 1 antiserum plus complement abolished their helper activity. We also demonstrated that purified T cell populations selectively triggerred IgG B cells. Selective triggering of IgG B lymphocytes by Tbc‐primed T cells may not be due to the influence of suppressor T cells supposedly present in Tbc‐primed cells since this selectivity was not affected by X‐irradiation of Tbc‐primed T cell populations which may inactivate suppressor T cells. Furthermore, passive transfer of Tbc‐primed cells into normal recipient mice, the condition which may detect the suppressor T cell effect much more sensitively in IgE production, or preimmunization with Tbc 2 weeks before, did not suppress primary anti‐DNP IgE antibody response to DNP‐PPD. Thus, the observations presented here are favorable to the concept of the presence of IgG class‐specific helper T lymphocytes. Furthermore, PPD‐reactive T cells from mice immunized with PPD itself exerted their helper function for triggering B cells of both IgE and IgG classes. This may also indicate that some of the components associated with Tbc other than PPD might negatively affect the development of PPD‐reactive helper T cells specific to the IgE class. The generation of such IgG‐specific T cell activity in the presence of Tbc will be discussed in the light of the T cell population involved in the regulation of antibody responses of different immunoglobulin classes.