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Complement Proteins and Macrophages
Author(s) -
Kawamoto Yasuko,
Ueda Masanaga,
Ichikawa Hidetaka,
Miyama Akio
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00529.x
Subject(s) - cycloheximide , complement factor b , lysozyme , microgram , biology , complement factor i , antibody , macrophage , microbiology and biotechnology , immunology , complement system , biochemistry , in vitro , protein biosynthesis
Cobra venom factor was used for the detection of factor B synthesized by mouse peritoneal macrophages. This method was shown to be specific for factor B assay by neutralization by antimouse factor B antibody. The amount of factor B in the culture supernatant, assessed by this method, was found to be dependent on the medium used for cultivation of macrophages. The addition of 25% L cell‐conditioned medium to minimal essential medium (LCM‐MEM) enhanced the production of factor B and also of lysozyme. Kinetic analysis in LCM‐MEM showed that factor B produced by 6 × 10 4 cells/cm 2 increased up to 72 hr and reached a plateau at 96 hr. The amounts of factor B and lysozyme produced in LCM‐MEM depended upon the number of macrophages. Production of factor B was completely inhibited by 1 μg of cycloheximide per ml and was restored by its removal.