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Studies on the Neutralization of Herpes Simplex Virus
Author(s) -
Yoshino Kamesaburo,
Isono Noriko,
Tada Aiko,
Urayama Mari
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00528.x
Subject(s) - herpes simplex virus , avidity , neutralization , virus , antibody , virology , neutralizing antibody , serial dilution , titer , biology , immunology , medicine , alternative medicine , pathology
A sample of late IgG from a rabbit hyperimmunized with herpes simplex virus was analyzed for neutralizing (N) and complement‐requiring neutralizing (CRN) antibodies. In a usual endpoint test, N and CRN titers were 1 : 40 and 1 : 160, respectively, but when virus‐IgG mixtures were incubated at 0 C overnight before addition of complement (C), an endpoint of 1 : 1280 was obtained. Virus sensitized at 0 C overnight required more C for inactivation than did sensitized virus formed earlier. Sensitization kinetic curve experiments employing a proper initial virus concentration, which permitted differentiation of sensitized viruses requiring different amounts of C, indicated that formation of sensitized virus detectable only with a relatively large amount of C proceeded slowly at IgG dilutions where the ordinary CRN antibody requiring a smaller amount of C was negligible. The results strongly suggested that the IgG sample contained slow‐reacting CRN (s‐CRN) antibody in excess of the hitherto known CRN antibody. As to the mechanism of formation of s‐CRN complexes, experiments failed to prove the occurrence of complexes initially insensitive to C, and it appears more likely that s‐CRN antibody has a comparatively low avidity for virus.