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Partial Purification, Physicochemical Characterization and Biological Function of a Rosette‐Decreasing Factor (RDF) Extracted from Guinea Pig Thymus
Author(s) -
Mabuchi Ayako,
Kanamori Sachio,
Yokomuro Kozo,
Kimura Yoshitami
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00508.x
Subject(s) - guinea pig , biology , concanavalin a , rosette (schizont appearance) , biochemistry , microbiology and biotechnology , dna synthesis , size exclusion chromatography , spleen , dna , immunology , endocrinology , in vitro , enzyme
A factor that decreases rosette formation between guinea pig T‐cells and rabbit red blood cells (RRBC) was extracted from the thymus of the guinea pig. The active factor could be extracted from the spleen as well as the thymus, but not from the liver or kidney. The active factor of the thymic extract was found in the precipitates produced by 80% saturated ammonium sulfate and it was separated from the water‐soluble fraction of the precipitates. The molecular weight of the partially purified substance was estimated to range between 10,000 and 30,000 by filtration through a diaflow membrane. From the studies on physicochemical characterization, it might be a heat‐resistant basic peptide probably bound to a ribonucleotide moiety. This factor reduced rosette formation between RRBC and guinea pig T‐cells, but did not reduce erythrocyte‐antibody‐complement rosette formation. This factor also inhibited mitogen (concanavalin A, phytohemagglutinin‐P)‐induced DNA synthesis of guinea pig lymphocytes and antigen‐induced DNA synthesis of sensitized guinea pig lymphocytes.