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Enrichment of C‐Type Virus mRNA from Immuno‐Chemically Separated Polyribosomes
Author(s) -
Trombley Lucy,
Wang CheiSuei
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00503.x
Subject(s) - polysome , messenger rna , biology , rna , virus , microbiology and biotechnology , affinity chromatography , biochemistry , virology , ribosome , enzyme , gene
A method for the isolation of mRNA from a c‐type virus‐positive cell (JLS‐V6) has been developed. This method consisted of (i) specific immunochemical precipitation of polyribosomes synthesizing viral proteins, (ii) extraction of the mRNA from the polyribosome/antigen/antibody complex, and (iii) separation of poly‐A containing RNA by affinity chromatography on oligo‐d(T)‐cellulose. Three size classes of virus specific poly‐A containing mRNA are identified and they are 35S, 20S–25S, and 10S–15S.