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Induction of Pre‐Early Nuclear Antigen(s) in HEL Cells Infected with Human Cytomegalovirus
Author(s) -
Tanaka Shigeaki,
Otsuka Masahiro,
Ihara Seiji,
Maeda Fumiko,
Watanabe Yasushi
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00462.x
Subject(s) - biology , antigen , immunoprecipitation , immunofluorescence , virus , human cytomegalovirus , microbiology and biotechnology , virology , gel electrophoresis , polyacrylamide gel electrophoresis , antibody , immunology , biochemistry , enzyme
Human cytomegalovirus (HCMV)‐specific nuclear antigen could be detected within 1 hr after infection in human embryo lung cells by the anticomplement immunofluorescence (ACIF) test. This antigen has been named the pre‐early nuclear antigen (PENA) in this paper. Serum absorption tests suggested that PENA is immunologically different from the early antigen and the major nuclear inclusion antigens detected by the indirect immunofluorescence test before and after viral DNA replication, respectively. PENA‐forming ability of the virus corresponded to its plaque forming ability. PENA formation was not affected by phosphonoacetate but was inhibited by the addition of inhibitors of RNA and protein syntheses or by UV‐irradiation of infecting virus, suggesting that the formation of PENA depends on the expression of infecting virus gene functions. Virus‐specific proteins were isolated by indirect immunoprecipitation from HCMV‐infected cells exposed to 35 S‐methionine. SDS‐polyacrylamide gel electrophoresis of the immunoprecipitate showed that at least two species of virus‐specific polypeptides with molecular weights of 70,000 and 30,000 were synthesized de novo within 3 hr after infection.