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In Vitro Immune Response of Human Peripheral Lymphocytes
Author(s) -
Kuritani Taro,
Hirano Toshio,
Kishimoto Tadamitsu,
Yamamura Yuichi
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00455.x
Subject(s) - biology , suppressor , immune system , cytotoxic t cell , in vitro , myeloid derived suppressor cell , microbiology and biotechnology , immunology , cancer , genetics
Con A‐stimulation of human peripheral T lymphocytes induced both suppressor and helper T cells. Con A‐generated suppressor T cells inhibited PWM‐indueed IgG and IgM production in PBL. Lower concentrations of Con A (0.5 μg/ml) or shorter incubation periods (6 to 24 hr) induced mainly helper T cells, while higher concentrations of Con A (10 μg/ml) or longer incubation periods (at least 48 hr) induced suppressor T cells. Con A‐generated suppressor T cells were sensitive to mitomycin treatment and exerted their suppressor function on the early phase of differentiation and/or proliferation of B cells but not on the final differentiation of B cells to Ig‐producing cells. The identity of the MHC was not required for the expression of suppressor function. Suppressor T cells competed with helper T cells in PWM‐induced Ig‐production of PBL. This experimental system can be applied to estimate the regulatory function of T cells in several disease states.