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Physico‐ and Immunochemical Properties of Staphylococcal Protein A Extracellularly Produced by a Set of Mutants from Staphylococcus aureus Cowan I
Author(s) -
Movitz Jan,
Masuda Shogo,
Sjöquist John
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00441.x
Subject(s) - mutant , antigenicity , biology , staphylococcus aureus , ouchterlony double immunodiffusion , protein a , radial immunodiffusion , microbiology and biotechnology , immunodiffusion , strain (injury) , mutant protein , antibody , genetics , bacteria , gene , anatomy , antiserum
Mutant strains of Staphylococcus aureus have been isolated using a simple cosedimentation technique. While the parental strain contains predominantly a cell‐bound protein A, the mutant strains exclusively produce extracellular protein A. The mutant forms of protein A all have lower molecular weights than that of protein A from the parental strain. They showed the same antigenicity as the parental protein A and gave similar reactivity with immunoglobulin to the parental one except for one mutant. A conspicuous spur was observed between the parental protein A and that produced by the mutant against normal dog serum in the micro‐Ouchterlony immunodiffusion test.