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Spontaneous Production of a C‐Type RNA Virus in a Cell Line Derived from Rat Glioma
Author(s) -
Igarashi Koichi,
Sasada Reiko,
Niiyama Yasuhiko,
Kozai Yoshio,
Sugino Yukio
Publication year - 1979
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1979.tb00435.x
Subject(s) - biology , rna , microbiology and biotechnology , dna , rna dependent rna polymerase , polymerase , virus , virology , murine leukemia virus , nucleic acid thermodynamics , cell culture , rna polymerase , gene , biochemistry , genetics
The spontaneous production of a rat C‐type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were: morphology typical of C‐type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase activity; viral core with a density of 1.28 to 1.30 g/ml; 70S RNA with dimer structure; and structural protein composed of mainly four polypeptides. Kinetical analysis of DNA‐DNA hybridization revealed that DNA sequences homologous to DNA transcripts of RNA of ACV were present in rat cells. RNA directed DNA polymerase of ACV partially cross‐reacted with antiserum to the polymerase of Rauscher murine leukemia virus. These data suggest that ACV is an endogenous C‐type RNA virus of rat origin.