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Effect of Double Infection of Cowpox Virus‐Infected Cells with Paramyxovirus (Sendai Virus) on Formation of Cowpox Virus‐Specific Cell Surface Antigen
Author(s) -
Tanaka Junji,
Ogura Hisashi,
Fukuda Shizuo,
Hatano Motoichi
Publication year - 1978
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1978.tb00430.x
Subject(s) - sendai virus , virology , biology , cowpox virus , virus , superinfection , infectivity , cowpox , syncytium , microbiology and biotechnology , vaccinia , biochemistry , gene , recombinant dna
The formation of cowpox virus‐specific cell surface antigen (CPV S‐ag) was significantly enhanced by double infection with HVJ (Sendai virus). Simultaneous double infection, superinfection with HVJ and superinfection with CPV of cells persistently infected with HVJ similarly enhanced the formation of CPV S‐ag, while pre‐infection with HVJ was ineffective. To be effective, cells must be infected at a m.o.i. of ≧ 1.0 and HVJ gene functions had to be expressed. The HVJ‐infected cell extracts had an ability to accelerate uncoating (or degradation) of CPV, causing an early increase and a subsequent decrease in the infectivity of CPV. This activity reached a maximum 4–6 hr after HVJ infection, the increase paralleling enhancement of the total activity of several cellular enzymes. Addition of puromycin abolished the increase of these activities and the formation of CPV S‐ag. Thus, the double infection with HVJ of CPV‐infected cells induces an enhancement of CPV S‐ag formation presumably as a consequence of activation of cellular enzymes which in turn accelerates uncoating of CPV.