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Induction of C‐Type Virus in Cell Lines Derived from Calf Form Bovine Lymphosarcoma
Author(s) -
Onuma Misao,
Okada KŌSuke,
Yamazaki Yooko,
Fujinaga Kei,
Fujimoto Yutaka,
Mikami Takeshi
Publication year - 1978
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1978.tb00421.x
Subject(s) - biology , virology , virus , bovine leukemia virus , cell culture , dexamethasone , in vitro , antigen , microbiology and biotechnology , immunology , genetics , endocrinology
For attempt to detect an etiological agent, cultures from bovine lymphosarcoma cases (adult form (ALS), calf form (CLS), and thymic form (TLS)) were maintained in vitro for over a 18 month period. In two cultures from ALS, bovine leukemia virus (BLV) antigen was constantly detected. On the other hand, BLV antigen remained negative in cultures from two CLS and one TLS cases up to 40 passages. The RNA dependent DNA polymerase activities in these cultures were also negative. Treatment of a culture from CLS (3178) originated from liver tumor with 5′‐iodo‐2′‐deoxyuridine (IdU) and dexamethasone (DXM) resulted in production of an agent serologically and morphologically similar to BLV and in alteration of cell morphology. No virus was detected in culture from TLS after treatment with IdU and DXM.