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Separation of RRBC‐RFC and Non‐Rosette Forming Cells (non‐RFC) of Guinea Pig T‐Cell Populations and Their Functional Difference
Author(s) -
Yokomuro Kozo,
Mabuchi Ayako,
Kimura Yoshitami
Publication year - 1978
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1978.tb00411.x
Subject(s) - biology , centrifugation , rosette (schizont appearance) , guinea pig , differential centrifugation , fraction (chemistry) , microbiology and biotechnology , antigen , cell , stimulation , immunology , chromatography , biochemistry , chemistry , endocrinology
Guinea pig lymph node cells suspension (LNC‐O) was filtered through a glass wool column and the effluent (LNC‐G) was further filtered through a nylon column. In this effluent (LNC‐NE) about 30 per cent of the lymphocytes was identified as non‐rosette forming cells (non‐RFC). The non‐RFC fraction was separated from LNC‐NE fraction by Ficol‐Conray specific gravity centrifugation of effluent cells reacted previously to rabbit red blood cells (RRBC). The upper layer after centrifugation, designated non‐RFC fraction, was separated. In this fraction 96% of the cells were lymphocytes and about 95% of them were non‐RFC, which lacked receptors for rabbit red blood cells (RRBC) or EAC and detectable surface immunoglobulin by conventional techniques. Though the response of the lymphocytes in the non‐RFC fraction to mitogenic (Con‐A, LPS) or antigenic stimulation was lower in comparison with that in RFC‐rich fraction, the response of non‐RFC to ConA exceeded the response to LPS. These facts suggest that at least a portion of the non‐RFC may be cells from the T‐cell line.