Premium
Speculation and C 2 Toxin Production by Clostridium botulinum Type C Strains Producing No C 1 Toxin
Author(s) -
Nakamura Shinichi,
Serikawa Toshihiko,
Yamakawa Kiyotaka,
Nishida Shoki,
Kozaki Shunji,
Sakaguchi Genji
Publication year - 1978
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1978.tb00409.x
Subject(s) - toxin , clostridium botulinum , microbiology and biotechnology , biology , spore , toxicity , trypsinization , population , biochemistry , chemistry , trypsin , medicine , environmental health , organic chemistry , enzyme
Abstract All of the 8 strains that were previously assumed to be nontoxigenic Clostridium botulinum type C were re‐examined for their toxigenicity and were demonstrated by trypsinization of the culture filtrates to produce C 2 toxin under improved cultural conditions. One per cent glucose added to trypticase peptone medium enhanced C 2 toxin production. The larger the spore population, the higher the C 2 toxicity and when spore population was smaller than a level of 10 4 /ml, no C 2 toxicity was demonstrated. The C 2 toxin was produced only during sporulation and not during vegetative growth.