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Nature of Antigenic Determinant of Serovar‐Specific Antigen of Leptospira interrogans Serovar hebdomadis
Author(s) -
Adachi Yoshikazu,
Yanagawa Ryo
Publication year - 1978
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1978.tb00400.x
Subject(s) - antigen , leptospira interrogans , biology , amino acid , antiserum , biochemistry , complement fixation test , serotype , microbiology and biotechnology , methionine , strain (injury) , leptospira , antibody , serology , genetics , anatomy , immunology
The nondialyzable delipidized serovar‐specific main antigen (NDTM antigen) of Leptospira interrogans serovar hebdomadis strain Hebdomadis (a variant which can grow in a synthetic medium) showed a strong inhibition of the complement fixation between the serovar‐specific main (TM) antigen of this strain and the homologous antiserum. The inhibitory effect of the NDTM antigen was completely lost by treating the antigen with proteolytic enzymes, and the fractions of TM antigen containing amino sugar, neutral sugars, and lipids did not show any inhibition of complement fixation, indicating that the antigenic determinant of this strain is related to proteins. NDTM antigen contained more hydrophilic amino acids than hydrophobic amino acids, whereas TM antigen contained more hydrophobic amino acids than hydrophilic amino acids. The amino acid compositions of NDTM antigens of hebdomadis strain Hebdomadis (variant) and kremastos strain Kyoto, which belonged to the same serogroup, were considerably similar. Difference was found in the amounts of methionine, arginine, lysine and glutamic acid.