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Cell Wall Autolysis in Log Phase Cells of Micrococcus lysodeikticus ( luteus ) *
Author(s) -
Monodane Toshihiko,
Matsushima Yoshio,
Hirachi Yoshiyuki,
Kotani Shozo
Publication year - 1978
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1978.tb00349.x
Subject(s) - autolysis (biology) , micrococcus luteus , biology , biochemistry , trypsin , microbiology and biotechnology , lysozyme , enzyme , chromatography , chemistry , escherichia coli , gene
Log phase cells of Micrococcus lysodeikticus (luteus) IFO 3333 autolyzed when incubated at 37 C in 0.01 m sodium‐phosphate buffer pH 7.5. The enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n ‐butanol, and proved to be an N‐acetylmuramyl‐ L ‐alanine amidase. The autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer.