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Comparative Studies on Cytopathic Effects Induced by Vesicular Stomatitis Virus in Two Cell Types
Author(s) -
Nishiyama Yukihiro,
Ito Yasuhiko,
Shimokata Kaoru,
Nagata Ikuya,
Kurachi Noriko,
Sugiura Yasuo
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00338.x
Subject(s) - vesicular stomatitis virus , biology , cell , cytopathic effect , virology , cell fusion , virus , immunofluorescence , cell culture , cell membrane , glycoprotein , microbiology and biotechnology , antibody , immunology , biochemistry , genetics
Infection of mouse L cells with vesicular stomatitis virus (VSV) leads to an extensive cell fusion, while porcine kidney stable (PS) cells infected with VSV show only cell rounding. Therefore, comparative morphological studies on the infection of the two cell lines were carried out using a transmission or scanning electron microscope and an immunofluorescence microscope. PS cells infected with VSV contrasted to L cells infected with the same virus in the following two points; (1) the principal site of VSV maturation was the intracytoplasmic vacuolar membrane in PS cells and the plasma membrane in L cells. However, it was found that viral glycoprotein was present on the cell surface of infected PS cells; (2) the morphological changes at the cell surface of infected PS cells occurred much earlier and were severer than those at the cell surface of infected L cells. From these observations, we discuss the possibility that the surface membrane of PS cells is too sensitive to the VSV‐induced cell damage to cause cell fusion.