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The Effect of the DNA‐Suppressing Factor (DSF) on Host DNA Synthesis in Synchronized Cell Cultures
Author(s) -
Minagawa Tomonori,
Fujii Nobuhiro,
Yamamoto Tomoko K.,
Iida Hiroo
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00332.x
Subject(s) - hela , biology , dna , dna synthesis , microbiology and biotechnology , measles virus , cell culture , cell , virology , biochemistry , genetics , measles , vaccination
Purified host DNA‐suppressing factor (DSF) produced into culture fluid of HeLa C‐9 cells infected with measles virus inhibited cellular DNA synthesis in HeLa cells. When purified DSF was added into cultures of synchronous HeLa cells at the early G 1 ‐phase, cellular DNA synthesis was irreversibly inhibited. However, DSF did not affect the stability of native double‐stranded DNA nor the chain‐elongation of single‐stranded DNA in cells of the S‐phase.