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Temperature Sensitive R Plasmids Isolated from Proteus Strains
Author(s) -
Odakura Yoshihiro,
Hashimoto Hajime,
Mitsuhashi Susumu
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00330.x
Subject(s) - plasmid , biology , proteus , escherichia coli , microbiology and biotechnology , bacteria , enterobacteriaceae , genetics , dna , gene
Out of 32 R plasmids isolated from Proteus strains, 17 were found to be temperature sensitive with respect to inheritance in E. coli cells. They were fi – and classified into incompatibility group T or V. Cells carrying T group Rms273 plasmid were temperature sensitive with respect to growth and conjugal transfer in both E. coli and Proteus . The V group YOR‐10 plasmid was stable in Proteus even at 42 C. However, the loss frequency of YOR‐10 plasmid in E. coli reached 100% after 4 hr of incubation at 42 C, in spite of stable inheritance at 25 C. Conjugal transfer of the YOR‐10 plasmid in E. coli was also strongly inhibited at 42 C. It has been concluded that instability of V group R plasmids in E. coli is due to their thermosensitive inheritance in the progeny cells at high temperatures.