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Macrophage Dependency of T‐lymphocyte Mitogenesis by Nocardia rubra Cell‐Wall Skeleton
Author(s) -
Sugimura Kazuhisa,
Uemiya Masanao,
Azuma Ichiro,
Yamawaki Mikio,
Yamamura Yuichi
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00318.x
Subject(s) - biology , t lymphocyte , macrophage , spleen , microbiology and biotechnology , interleukin 2 , t cell , lymphocyte , immunology , biochemistry , antigen , cytokine , in vitro , immune system
The mitogenic activity of the cell‐wall skeleton (CWS) of Nocardia rubra on purified splenic T‐cells (thymus‐derived lymphocytes) was investigated. N. rubra CWS showed remarkable mitogenic activity on normal spleen cells of C57BL/6J mice at concentrations ranging from 10 to 100 μg per milliliter of culture medium, while, on purified splenic T‐cells, N. rubra CWS did not act as a mitogen at any concentration. However, mitogenic activity of N. rubra CWS on T‐cells was restored if purified splenic T‐cells were reconstituted with X‐irradiated peritoneal exudate cells (macrophages). The above results suggest the necessity of macrophages for T‐lymphocyte activation by N. rubra CWS as well as PHA‐P or Con A.