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Intracellular Development of Membrane Protein of Influenza Virus
Author(s) -
Maeno Koichiro,
Yoshii Saiji,
Yoshida Tetsuya,
Iinuma Masao,
Kawamoto Yasuko,
Matsumoto Toshisada
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00308.x
Subject(s) - cytoplasm , biology , hemagglutinin (influenza) , antigen , antiserum , virus , virology , intracellular , clone (java method) , nucleoprotein , microbiology and biotechnology , staining , viral matrix protein , biochemistry , immunology , dna , genetics
The intracellular development of membrane protein (MP) of influenza A virus was investigated by immunofluorescent staining. Monospecific antiserum was prepared by immunizing rabbits with MP eluted from SDS‐polyacrylamide gels of SDS‐disrupted NWS virions. In the productive infection in clone 1‐5C‐4 cells, MP antigen was first detected over the whole cell at 4 hr after infection, concomitantly with the appearance of hemagglutinin (HA) antigen in the cytoplasm, and bright nuclear fluorescence was then observed. Nucleoprotein (NP) antigen was detected in the nucleus prior to the appearance of fluorescence of MP antigen and thereafter the cytoplasmic fluorescence developed. Late in infection, all of these three antigens were observed predominantly in the cytoplasm with stronger fluorescence at the cell surface. Essentially similar findings were obtained in the abortive infections in L cells and BHK cells. The above results suggest that the membrane protein of influenza A virus is present in the nucleus as well as in the cytoplasm of infected cells.