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Induction and Properties of DNP‐Specifie T Cells in Mice Sensitized to DNP‐Coupled Mycobacterium
Author(s) -
Hirai Yoshikatsu,
Kishimoto Tadamitsu,
Suemura Masaki,
Yamamura Yuichi
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00303.x
Subject(s) - biology , microbiology and biotechnology , mycobacterium , bacteria , genetics
Splenic T lymphocytes from mice sensitized to 100 μg of DNP‐coupled mycobacterium (DNP‐Tbc) showed in vitro proliferative response against DNP‐or TNP‐conjugated protein antigens. The increased uptake of 3 H‐thymidine induced by DNP‐HSA was partially inhibited by the addition of 10 –4 M DNP‐EACA. DNP‐AECM‐Ficoll did not induce any significant proliferative responses in DNP‐Tbc‐primed T cell population. However, priming with DNP‐Tbc augmented anti‐DNP IgG antibody response induced with DNP‐Ficoll. The augmentation of IgG response was not due to the presence of DNP‐primed B cells or anti‐DNP antibody. The results showed that the priming with DNP‐Tbc induced DNP‐reactive T helper cells which could be triggered with DNP‐Ficoll. The possible role of mycobacterium in the induction of hapten‐specific T helper cells is discussed.