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Restitution of Hemagglutinating Activity to Spikeless Particles of HVJ (Sendai Virus) by Glycoprotein Components of Newcastle Disease Virus
Author(s) -
Iinuma Masao,
Maeno Koichiro,
Matsumoto Toshisada,
Hoshino Munemitsu
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00302.x
Subject(s) - sendai virus , newcastle disease , trypsin , glycoprotein , virus , biology , centrifugation , infectivity , virology , enzyme , antiserum , biochemistry , microbiology and biotechnology , antibody , immunology
Spikeless particles of HVJ (Sendai virus) lacking in hemagglutinating (HA) activity were obtained by enzymatic digestion of virions with trypsin followed by centrifugation through a sucrose gradient. When they were mixed with glycoprotein components of Newcastle disease virus (NDV) obtained by treatment of purified virions with deoxycholate (DOC), the mixture showed hemagglutination reaction, which was inhibited by anti‐NDV serum, but not by anti‐HVJ serum. Sedimentation profile of the HA active agents was then examined by centrifugation of the mixture of spikeless particles of HVJ (labeled with 3 H‐uridine) and glycoproteins of NDV (labeled with 14 C‐amino acid mixture). The results showed that the peak of HA activity had both of the radioactivities, and that the sedimentation rate of the HA was faster than that of spikeless HVJ but slower than that of intact HVJ. Electron micrographs of such HA active structures showed that they were morphologically closely similar to intact virion of HVJ, although they had neither hemolytic activity nor infectivity. The mixture of spikeless HVJ and glycoproteins of HVJ or NDV which were removed from virions by proteolytic enzymes, on the other hand, did not show any detectable hemagglutinating activity.