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Toxin Isolation from a Kanagawa‐Phenomenon Negative Strain of Vibrio parahaemolyticus
Author(s) -
Sochard M.R.,
Colwell R.R.
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00285.x
Subject(s) - vibrio parahaemolyticus , microbiology and biotechnology , toxin , biology , bacteria , vibrionaceae , trypsin , enterotoxin , strain (injury) , hemolysin , enzyme , biochemistry , escherichia coli , virulence , genetics , anatomy , gene
Production of a toxin by Vibrio parahaemolyticus Kanagawa‐phenomenon negative strains was examined. Ammonium sulfate fractions of broth culture filtrates were dialyzed, concentrated by lyophilization, and tested for toxic effects by mouse intraperitoneal injection. One fraction, which we think is a toxin, was isolated from a broth culture filtrate of V. parahaemolyticus FC 1011 (a Kanagawa‐phenomenon negative strain) and consistently produced lethal effects in mice at high concentrations and diarrhea in lower concentrations. The toxin was assayed for mouse LD50 and ability to produce diarrhea via forced feeding in mice. V. parahaemolyticus FC 1011 toxin was found to be protein, to be inactivated by heat or trypsin hydrolysis, and to produce positive skin permeability reactions in rabbits. However, it failed to induce fluid accumulation in ligated ileal loops in rabbits.