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Effect of Various Reagents on the Susceptibility of Target Cells to the Cytotoxicity of Guinea Pig Lymphotoxin (GLT) 1
Author(s) -
Sawada JunIchi,
Kishie Takao,
Osawa Toshiaki
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00277.x
Subject(s) - cytotoxicity , puromycin , lymphotoxin , biology , colchicine , guinea pig , biochemistry , cytotoxic t cell , microbiology and biotechnology , protein biosynthesis , in vitro , endocrinology , genetics , receptor
The cytotoxic activity of guinea pig lymphotoxin (GLT) was increased when the target cells were treated with the following metabolic inhibitors: puromycin, actinomycin D, NaN 3 , NaF, and 2,4‐dinitrophenol, whereas calf serum, N‐acetyl‐ D ‐galactosamine, colchicine, and vinblastin inhibited GLT‐cytotoxicity. Puromycin, an inhibitor of protein synthesis, exhibited the most potent enhancing effect on GLT‐cytotoxicity. It seems likely that there are certain cellular metabolic processes depending on cellular protein synthesis which antagonize GLT‐cytotoxicity. Taking advantage of this effect of puromycin, a time‐saving assay method for GLT‐cytotoxicity was developed.