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Heterogeneity of Staphylococcal Enterotoxin A on Isoelectric Focusing and Disc Electrophoresis
Author(s) -
Yamada Sumio,
Igarashi Hideo,
Terayama Takeshi
Publication year - 1977
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1977.tb00273.x
Subject(s) - isoelectric focusing , isoelectric point , electrophoresis , toxin , enterotoxin , chromatography , gel electrophoresis , biology , staphylococcus aureus , microbiology and biotechnology , chemistry , biochemistry , bacteria , enzyme , escherichia coli , genetics , gene
Heterogeneity of purified staphylococcal enterotoxin A, obtained from a culture supernatant of Staphylococcus aureus , strain 13N‐2909, was demonstrated by isoelectric focusing. The toxin was composed of three immunologically identical fractions with isoelectric points of 6.5, 7.0 and approximately 8.0. Heterogeneity of the toxin was also shown by disc electrophoresis. At pH 8.0 and 9.4 two major bands and a faint minor band were observed, while at pH 4.3 only one band was observed. The faster‐moving band for the anode in disc electrophoresis at pH 9.4 was found to correspond with the pI 6.5 component from isoelectric focusing, while the slower‐moving band corresponded with the pI 7.0 component. From the results of the electrophoretic migration tests of the toxin, the components corresponding to the two major bands found in disc electrophoresis at pH 9.4 were considered to be charge isomers.