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Binding of Tobacco Mosaic Virus to Membrane Material Isolated from Tobacco Leaves
Author(s) -
Kiho Yukio,
Shimomura Toru
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb01022.x
Subject(s) - tobacco mosaic virus , pronase , chemistry , membrane , biochemistry , tobacco leaf , biology , botany , virus , enzyme , virology , trypsin , agricultural engineering , engineering
Binding of tobacco mosaic virus (TMV) to disrupted tobacco leaf membrane was studied. Membrane isolated from tobacco leaves was treated successively with (NH 4 ) 2 SO 4 , Li‐diiodosalicylate and then pronase. TMV‐binding substance was thus isolated in a soluble form. From enzymatic digestion experiments, it was suggested that the binding substance was composed of lipid and carbohydrate.