Premium
Purification of Host DNA Synthesis‐Suppressing Factor (DSF) Produced by Infection with Measles Virus
Author(s) -
Yamamoto Tomoko K.,
Minagawa Tomonori,
Iida Hiroo
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb01018.x
Subject(s) - measles virus , hela , sephadex , isoelectric point , isoelectric focusing , ammonium sulfate precipitation , microbiology and biotechnology , virus , biology , dna , chemistry , size exclusion chromatography , biochemistry , virology , in vitro , measles , enzyme , vaccination
Host DNA synthesis‐suppressing factor (DSF) produced into culture fluid of cloned HeLa cells (HeLa C‐9) infected with a small plaque variant of Toyoshima strain of measles virus was purified by precipitation with ammonium sulfate, chromatography on CM‐cellulose and DEAE‐cellulose, and gel‐filtration on Sephadex G‐100 and G‐200. The specific activity of the finally purified DSF was 302 units/mg of protein representing approximately 300‐fold purification. The molecular weight of DSF was estimated to be about 55 000. By isoelectric focusing, two kinds of DSF having isoelectric points of 4.24 and 5.24 were detectable. The purified DSF was able to suppress host DNA synthesis of HeLa cells, continuous human lymphoid cells (NC‐37), mouse L cells and Meth‐A cells derived from an ascitic tumor of the mouse. The activity of the purified DSF was inactivated by heating at 56 C for 30 min or by treatment with trypsin.