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Persistent High Numbers of Clostridium perfringens in the Intestines of Japanese Aged Adults
Author(s) -
Yamagishi Takayoshi,
Serikawa Toshihiko,
Morita Ryoko,
Nakamura Shinichi,
Nishida Shoki
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb01005.x
Subject(s) - clostridium perfringens , feces , serotype , agar , microbiology and biotechnology , biology , toxin , agar plate , bacteria , genetics
TSN agar was applicable for enumeration of Clostridium perfringens in fecal samples of adults but not in those of infants. It was demonstrated using TSN agar that some healthy aged adults had persistently carried C. perfringens at levels ranging from 10 7 to 10 9 , while some others ranged from 10 3 to 10 6 per ml volume of fecal sample although all of these adults had the same diets. In the test for agglutinability of isolates of C. perfringens collected from two elderly adults, a younger adult and a baby, it was demonstrated that most of the isolates obtained from an aged adult of high levels for 19 months belonged the same serotype, while rapid alteration of serotypes could be observed in three other persons with high or low levels. In spite of as many as 10 9 C. perfringens per ml of feces, no trace of α‐toxin could be detected in the fecal samples. In in vitro tests, fecal suspension suppressed the production of α‐toxin although it allowed the organism to grow sufficiently.