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In Vitro Studies on the Mechanism of Acquired Resistance to Tuberculous Infection
Author(s) -
Muraoka Shizuko,
Takeya Kenji,
Nomoto Kikuo
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb01002.x
Subject(s) - immune system , bacilli , lymph node , antigen , macrophage , mycobacterium tuberculosis , microbiology and biotechnology , tubercle , biology , in vitro , adjuvant , immunology , immunity , lymph , tuberculosis , t cell , medicine , pathology , bacteria , biochemistry , genetics
Immune lymph node cells were obtained from mice immunized with bovine gamma globulin (BGG) in complete Freund's adjuvant or allogeneic MH134 tumor cells. They showed the capacity of conferring bactericidal activity on macrophages infected with Mycobacterium tuberculosis , H37Rv, when they were incubated on macrophage monolayers together with the corresponding antigen, i.e., BGG or solubilized cellular antigen of the tumor cells. However, such capacity was lower than that of tubercle bacilli‐immune lymph node cells. Culture supernatants were harvested after incubation of tubercle bacilli‐immune, BGG‐immune or allogeneic tumor‐immune lymph node cells with the corresponding antigen for 24 hr. Macrophages were altered so as to suppress intracellular bacillary growth when macrophage monolayers were exposed to the supernatants for more than 2 days. When normal lymph node cells were incubated on normal macrophage monolayers together with a mitogen such as PHA or concanavalin A, growth of tubercle bacilli within the macrophages was slightly but difinitely suppressed. The mechanism of elicitation of cellular immunity to the infection with tubercle bacilli is discussed on the basis of results presented in this and the preceding paper.