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Studies on Delayed Hypersensitivity of Antigens Isolated from Mycoplasma pneumoniae Cells
Author(s) -
Suzuki Masuko,
Hayashi Yoshiro,
Arai Sumio,
Kumagai Katsuo
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb00974.x
Subject(s) - mycoplasma pneumoniae , antigen , microbiology and biotechnology , mycoplasma , virology , immunology , biology , medicine , pneumonia
Cells of Mycoplasma pneumoniae Mac strain were fractionated into acetone‐soluble and insoluble fractions. Acetone‐insoluble fractions were digested with pronase and further purified by chromatography on Sephadex G‐75, yielding three water‐soluble fractions which were free from lipid and consisted mainly of polysaccharide‐protein complex. All these water‐soluble fractions possessed eliciting antigenicity to delayed hypersensitivity for M. pneumoniae as measured by skin reactions and macrophage migration inhibition tests, but not to complement‐fixing antibodies. In contrast, the acetone‐soluble fraction was reactive with the complement‐fixing antibodies but not for the delayed hypersensitivity.