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Strain Differences in the Immunogenicity of Aggregated Human IgG and the Adjuvant Action of Lipopolysaccharide on the Low‐Responder Strain of Mice
Author(s) -
Fujiwara Michio,
Fujiwara Shigeko,
Yoshizaki Chiho,
Awaya Akira
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb00920.x
Subject(s) - immunogen , lipopolysaccharide , immunogenicity , spleen , adjuvant , strain (injury) , antibody , antigen , chemistry , thymidine , immunology , microbiology and biotechnology , biology , medicine , monoclonal antibody , in vitro , biochemistry
Strain differences in the antibody response to human IgG (HGG) were observed when aggregated HGG was injected intravenously. Lipopolysaccharide (LPS) administered subsequently markedly enhanced the antibody response to HGG in low responder C57BL/6 mice as compared with that in high responder DDD, C3H/He or (C57BL/6 × DDD)F 1 mice. Aggregate‐free preparation of HGG at a dose of 0.5 mg induced immunological tolerance in all strains of mice tested. LPS injected subsequently converted tolerogenic, aggregate‐free HGG into immunogen in DDD mice but not in C57BL/6 mice. To determine the correlation between adjuvanticity and mitogenicity of LPS, spleen cells from normal mice were cultured in the presence of LPS and 3 H‐thymidine uptake was measured. Spleen cells of DDD mice incorporated three times as much 3 H‐thymidine as those of C57BL/6 mice. There seems no strong correlation between both activities of LPS. The data obtained are discussed in terms of strain differences in the macrophage function for processing the antigen.