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Immunochemical Studies on Bacterial Blood Group Substances
Author(s) -
Kishi Koichiro,
Iseki Shoei
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb00916.x
Subject(s) - fucose , polysaccharide , hydrolysis , chemistry , residue (chemistry) , biochemistry , borohydride , acid hydrolysis , escherichia coli , chromatography , galactose , gene , catalysis
Blood group H‐active polysaccharide has been prepared from “smooth” strain Escherichia coli 2B‐V by Freeman's method. a ‐Fucosidase derived from Bacillus fulminans caused the liberation of fucose from this polysaccharide, together with concomitant loss of blood group H activity. The results of quantitative microanalysis, borohydride reduction, the Morgan‐Elson reaction and enzymic hydrolysis with β‐galactosidase using isolated oligosaccharides obtained by partial acid hydrolysis indicated that the O‐specific side chain of the polysaccharide has a pentasaccharide unit which is β‐d‐Gal‐(1→3)‐d‐GalNAc‐(1→3)‐d‐GalNAc‐Fuc with a D ‐glucose residue bound at some undetermined point on this structure. It was considered that terminal non‐reducing fucose of the polysaccharide was liberated by partial acid hydrolysis.