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Ultrastructure and Chemical Analysis of the Cell Wall of Pythium debaryanum 1
Author(s) -
Yamada Michihisa,
Miyazaki Toshio
Publication year - 1976
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1976.tb00913.x
Subject(s) - ultrastructure , cell wall , polysaccharide , glucan , amorphous solid , reagent , electron microscope , chemistry , biology , biochemistry , crystallography , botany , organic chemistry , physics , optics
The ultrastructure and component polysaccharides of the cell wall of Pythium debaryanum IFO‐5919 were investigated. From results obtained by means of acid, alkali, Schweitzer reagent and β‐1, 3‐glucanase treatments and electron microscopy, it was concluded that 1) the acid‐extracted fraction was a 1,3‐linked branched glucan, 2) the alkali‐extracted fraction was a mixture of 1,3‐, 1,6‐, and 1,3,6‐linked highly branched two glucans, 3) the Schweitzer reagent‐extracted fraction was a β‐1, 4‐linked glucan, 4) the cell wall was constructed from two types of cullulosic microfibrils, as a frame and as a finer network, and amorphous β‐1, 3‐glucan including β‐1, 6‐linkage, 5) cellulosic microfibrils were covered by matrix material consisting of a mixture of amorphous β‐1, 3‐linked and β‐1, 6‐linked branching glucans.