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Synchronous Cell Differentiation in Caulobacter crescentus
Author(s) -
Iba Hideo,
Fukuda Akio,
Okada Yoshimi
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00960.x
Subject(s) - caulobacter crescentus , biology , bacteria , microbiology and biotechnology , rna , growth medium , population , bacterial growth , cell division , cell growth , cell , cell culture , biochemistry , genetics , gene , bacterial protein , demography , sociology
The growth of a stalked bacterium, Caulobacter crescentus , has been synchronized easily and reproducibly by a new method. When this bacterium is grown to a late log phase in nutrient broth at 30 C with aeration, swarmer cells are accumulated in the culture to 80% of the whole cell population. When this culture is inoculated into fresh pre‐warmed broth at twentyfold dilution, it immediately initiates synchronous cell growth. Simultaneously, synchronous cell differentiation is monitored by the susceptibility of the cells to RNA phage infection. The swarmer cells accumulated in the late log phase of growth possess nearly the same susceptibility to RNA phage infection as those in the early log phase of growth while RNA phage‐adsorbing capacity is lower in such swarmer cells. It is suggested that the swarmer cells accumulated in the late log phase of growth have lost some pili.

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