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R Factor‐Mediated Resistance to Aminoglycoside Antibiotics in Pseudomonas aeruginosa
Author(s) -
Sagai Hitoshi,
Krcmery V.,
Hasuda Katsumi,
Iyobe Shizuko,
Knothe H.,
Mitsuhashi Susumu
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00958.x
Subject(s) - carbenicillin , pseudomonas aeruginosa , microbiology and biotechnology , kanamycin , streptomycin , gentamicin , aminoglycoside , chemistry , phosphotransferase , escherichia coli , ampicillin , antibiotics , drug resistance , acetyltransferase , tetracycline , biology , enzyme , biochemistry , bacteria , genetics , acetylation , gene
Conjugal transferability of drug resistance was examined, in eleven Pseudomonas aeruginosa strains which were isolated in Frankfurt. Four R factors were demonstrated from three strains using P. aeruginosa as recipients but they were nontransferable to Escherichia coli K12. Two R factors, i.e., R ms146 and R ms147 , mediated resistances to tetracycline (TC), streptomycin (SM), sulfanilamide (SA), kanamycin (KM), lividomycin (LV), gentamicin C complex (GM) and 3′,4′‐dideoxykanamycin B (DKB). They mediated the formation of aminoglycoside‐inactivating enzymes, i.e., SM phosphotransferase, SM adenylyltransferase, KM and LV phosphotransferase 1, and GM and DKB 6′‐ N ‐acetyltransferase. TC resistance conferred by these R factors was due to impermeability of the drug. P. aeruginosa Ps 142 carried two kinds of R factor in one cell, R ms148 (SM) and R ms149 (SM·SA·GM·CPC) (CPC, carbenicillin). R ms148 (SM) was transferable at a high frequency of 10 –1 and mediated the formation of SM phosphotransferase. R ms149 mediated the formation of drug‐inactivating enzymes, i.e., GM 3‐ N ‐acetyltransferase and β‐lactamase, but did not inactivate SM. SM resistance was probably due to impermeability of the drug.